RNeasy Plus Kits is next generation QIAGEN RNA extraction kit, combining high-quality RNA purification with effective gDNA removal with unique gDNA Eliminator columns or plates. Total RNA of high purity and yield is obtained from a wide range of cultured cells and easy-to-lyse tissues. The RNeasy Plus Mini Kit purifies up to 100 µg total RNA (>200 nt) from a single extraction with efficient gDNA removal. The kit can be automated on the QIAcube Connect and samples can be conveniently stabilized using RNAprotect Tissue Reagent or Allprotect Tissue Reagent and efficiently disrupted using a TissueRuptor II or TissueLyser II or LT system (available separately).
RNeasy Plus Mini standard protocols can also be executed using the TRACKMAN Connected system, paired with PIPETMAN M Connected pipettes, both from Gilson. The TRACKMAN Connected system guides researchers through the RNeasy Plus Mini protocols while automatically adjusting the Bluetooth-enabled PIPETMAN M Connected pipette settings. Each step of the protocol execution is recorded to accelerate reporting by generating a comprehensive run report.
Features
- Efficient gDNA removal with unique gDNA Eliminator columns or plates (no need for DNase)
- High-quality total RNA in minutes using fast and simple extraction protocols
- Phenol-free RNA isolation
- High-throughput processing in 96-well format
- Ideal for sensitive applications such as real-time RT-PCR and RNA-seq