RNeasy Plus Kits are next‑generation RNA extraction kits from QIAGEN that combine high‑quality RNA preparation with effective removal of gDNA through unique gDNA eliminator columns or plates. High‑purity and high‑yield total RNA is obtained from a wide range of cultured cells and easily sectionable tissues. The RNeasy Plus Mini Kit purifies up to 100 µg total RNA (>200 nt) from a single extraction with efficient gDNA removal. The kit can be automated on the QIAcube Connect, and samples can be conveniently stabilized with the RNAprotect Tissue Reagent or the Allprotect Tissue Reagent and efficiently lysed with the TissueRuptor II or the TissueLyser II or LT System (available separately).
RNeasy Plus Mini standard protocols can also be performed with the TRACKMAN Connected System in conjunction with the PIPETMAN M Connected Pipettes, both from Gilson. The TRACKMAN Connected System guides researchers through the RNeasy Plus Mini protocols and automatically adjusts the settings of the Bluetooth‑enabled PIPETMAN M Connected Pipette. Each step of the protocol execution is recorded to accelerate reporting by creating a comprehensive run report.
Features
- Efficient gDNA removal with unique gDNA eliminator columns or plates (no DNase required)
- High‑quality total RNA in minutes with fast and simple extraction protocols
- Phenol‑free RNA isolation
- High‑throughput processing in 96‑well format
- Ideal for sensitive applications such as real‑time RT‑PCR and RNA‑seq