T4 DNA Ligase (Fast), 1,000 U

MCE T4 DNA Ligase (Fast) is produced by Escherichia coli carrying a T4 phage, catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.

The enzyme joins DNA fragments with either cohesive or blunt termini, repairs single-strand nicks in duplex DNA, RNA or DNA/RNA hybrids, but it is inactive against single-stranded nucleic acids.

It is suitable for labeling RNA 3'-ends, cyclizing RNA and DNA oligonucleotides and cloning cDNA. The T4 DNA Ligase requires ATP as a cofactor, completes viscous end connections in just 10 min at room temperature. Simple handling for fast and efficient connections.

Storage: -20℃