NutriFreez® D10 Cryopreservation Medium

NutriFreez® D10 Cryopreservation Medium is a ready-to-use, animal component-free, serum-free, and protein-free cell freezing solution optimized for multiple cell lines. When culturing cells in a serum-free environment, it is essential to also maintain serum-free conditions during cryopreservation.

NutriFreez® D10 Cryopreservation Medium has been demonstrated to result in high rates of cell viability, proliferation, adherence (in relevant lines), and bioactivity/ expression following freezing and thawing. Superior results were obtained both in comparison with serum-containing freezing media as well as competing serum-free products, making this an ideal product for both serum-containing and serum-free applications.

NutriFreez® D10 Cryopreservation Medium has been extensively tested on multiple cell types, including:

• Human Mesenchymal Stem Cells (hMSC), from various sources (e.g. bone marrow (BM-MSC), adipose tissue (AT-MSC), umbilical cord tissue (UC-MSC) derived and dental pulp tissue (hMSC-DP)
• Human Embryonic Stem Cells (hESC), Induced Pluripotent Stem Cells (iPSC)
• Human Peripheral Blood Mononuclear Cells (PBMC's)
• Human Endothelial Cells (EC)
• T cells, including Chimeric Antigen Receptors (CART) cells and Tumor-Infiltrating Lymphocytes (TIL's)
• Neuron Cells
• Hybridomas
• CHO cells
• Vero Cells
• Multiple mammalian cell lines: MRC-5, HEK-293, HepG2, HeLaBSC-1, BGM3T3, MA-10BHK-21 as well as other extremely sensitive cell types

NutriFreez® D10 Cryopreservation Medium Features

• Cryoprotective formulation designed to minimize dehydration effects
• Effective maintenance of cell viability, adhesion, and bioactivity
• Ready-to-use solution, simple protocol
• Suitable for use in cell banks and biopharmaceutical applications
• Animal component-free, serum-free, protein-free solution
• Manufactured in accordance with applicable cGMP guidelines
• Drug Master File available

Results of a cryopreservation comparison performed at the Research and Development Center for Cell Therapy (Japan) on human pluripotent stem cells (hESC and hiPSC) show that NutriFreez® D10 Cryopreservation Medium* presents best recovery rate for hPSCs after thawing (Nishishita et al. 2015). Furthermore, an evaluation of different cryopreservation agents for mesenchymal stem cells (MSC) performed at Ottawa Hospital Research Institute (Canada) shows that MSC cryopreserved in NutriFreez. D10 Cryopreservation Medium exhibit the best post-thaw viability (Salkhordeh et al. 2018)

* NutriFreez® brand replaces the brand CryoStem™

Nishishita, Naoki et al. "An effective freezing/thawing method for human pluripotent stem cells cultured in chemically-defined and feeder-free conditions”, American journal of stem cells, 4, 38-49, 2015

Salkhordeh, Mahmoud. et al. “Evaluation of different cryopreservation agents for mesenchymal stem cell as final study product”, Cytotherapy 20, S50, 2018